Silver Nanoparticles Illuminated Under a Darkfield Microscope

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This image was captured using a hyperspectral microscope (CytoViva, Auburn, AL). The different scattered colors provide information about the size and shape of the nanoparticles, and it has been shown that blue, green, yellow, and red correlate with sphere, pentagon, rounded-triangle, and triangle shaped particles, respectively (Mock et al., 2002). This data can be stored in a software program to later identify silver nanoparticles with specific properties within more complex environments, such as cells.